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KMID : 0355820220430010075
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Korean Journal of Oral Anatomy
2022 Volume.43 No. 1 p.75 ~ p.85
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Heat-inactivated Weissella cibaria CMU Downregulates the mRNA Expression of Proinflammatory Cytokines and Matrix Metalloproteinases in Porphyromonas gingivalis Lipopolysaccharide-Stimulated Human Gingival Fibroblasts
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Kim Min-Jeong
You Yong-Ouk
Kim Hyun-Jin
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Abstract
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Weissella cibaria CMU (oraCMU) is reported as one of the probiotics that promote oral health. Various periodontal pathogens, including Porphyromonas gingivalis (P. gingivalis), can cause periodontal tissue destruction and periodontal disease. P. gingivalis infection in human gingival fibroblasts (HGFs) induces the expression of proinflammatory cytokines and enhances the production of matrix metalloproteinases (MMPs). The present study investigated the inhibitory effects of heat-inactivated oraCMU on the mRNA expression of proinflammatory cytokines and MMPs in P. gingivalis LPS-stimulated HGFs. Cell viability was performed using the MTS assay. The mRNA expression of proinflammatory cytokines, such as interleukin-1β (IL-1β) and, IL-6, tumor necrosis factor-α (TNF-α), and MMPs, such as MMP-1 and MMP-3, was examined by reverse transcription-quantitative PCR (RT-qPCR). Heat-inactivated oraCMU dose-dependently inhibited the mRNA expression of IL-1β, IL-6, and TNF-α in P. gingivalis LPS-stimulated HGFs. Also, the mRNA expression of MMP-1 and MMP-3 dose-dependently decreased. These results suggest that heat-inactivated oraCMU could be an effective probiotic for preventing periodontitis.
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KEYWORD
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Weissella cibaria CMU, Matrix metalloproteinase, proinflammatory cytokines, Porphyromonas gingivalis, human gingival fibroblasts
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